Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions
نویسندگان
چکیده
منابع مشابه
Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions.
Amino-functionalized macroporous silica foam (NH2 -MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the functionalized NH2 -MOSF into the protein and trypsin solutions without altering the bulk pH or preloading the enzymes on the materials. With thi...
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Carbon dioxide (CO2) adsorption on unfunctionalized and amino-functionalized SBA-3 materials are investigated and compared in this study. The synthesized materials are characterized by various techniques such as X-ray diffraction (XRD), Brunauer-Emmet-Teller (BET) method, Fourier transform infrared (FT-IR) and Scanning electron microscopy (SEM).The isotherms of these materials have been measure...
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Tryptic digestion of proteins in trypsin loaded porous silica has been shown to be highly efficient. Enzymatic silica-reactors were prepared by immobilizing trypsin into macroporous ordered siliceous foam (MOSF) and into mesoporous SBA-15 silica which has a smaller pore size. The tryptic products from the silica reactors were analyzed by matrix-assisted laser desorption ionization time-of-fligh...
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The peptides produced by the tryptic digestion of S-aminoethylated bovine pancreatic ribonuclease support the generally accepted amino acid sequence for this enzyme and also demonstrate the usefulness as well as some special limitations of the aminoethylation procedure. The relative extents of hydrolysis at different peptide bonds were estimated from the yields of purified peptides. Under the c...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2013
ISSN: 1615-9853
DOI: 10.1002/pmic.201300108